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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Matrix Biol. 2009 Jul 28;28(8):463–469. doi: 10.1016/j.matbio.2009.07.005

Table 3.

Effect of glycosylation on TIMP-3 and N-TIMP-3 inhibition of ADAMTS4-2 and ADAMTS5-2.

N-TIMP-3 Kia TIMP-3 Kia
glycosylated b non-glycosylated c glycosylated b non-glycosylated d
ADAMTS5-2 0.35 ± 0.03 0.32 ± 0.12 0.72 ± 0.17 1.37 ± 0.4
ADAMTS4-2 0.18 ± 0.02 0.79 ± 0.02 0.22 ± 0.07 0.20 ± 0.08
a

Ki values (nM) are expressed as mean ± standard deviation (n=4).

b

Glycosylated TIMP-3 and N-TIMP-3 were expressed in HEK293 cells.

c

Non-glycosylated N-TIMP-3 was expressed in E. coli.

d

Non-glycosylated TIMP-3 was expressed in HEK293 cells treated with 5 μg/ml tunicamycin.