Fig. 6.
PYR1 molecular surface color-coded by electrostatic potential. ABA-induced changes in subunit orientation produce conformational changes in the disk-shaped PYR1 dimer at the (A) interacting lids (top), (B) concave sides, aligned as in Fig. 5 (D and E), and (C) the cleft (bottom) between C-terminal helices. Conformational changes of charged residues in the Leu-Lock (Glu114 and Arg116) and Recoil (Glu149, Glu153, Asp154, Asp155 and Arg157) motifs of each subunit reduce electrostatic surface potential upon lid closure (A).