Figure 4.

Pharmacological blockade of 12-LO abolishes LTCC-LTP. LTCC-dependent TBS-LTP in wild-type mice studied in the continuous presence of 50 μm d-APV was fully inhibited by 10 μm PD146176. Slices were preincubated in PD146716 for 2.5 h before experiment. Mean fEPSP amplitude was measured as percentage of baseline, averaged over a 5 min window 60 min after TBS. TBS in presence of d-APV (black) enhanced the fEPSP to 138 ± 3.45% of baseline (n = 5). In the presence of PD146176 plus d-APV (blue), TBS caused no significant change in fEPSP over baseline (96.6 ± 6.37%; n = 6; p < 0.0005 with unpaired Student's t test). The black bar indicates presence of drugs. Inset shows sample fEPSP traces before (solid line) and 60 min after (dashed line) LTP induction. Error bars represent ±SEM. The asterisks indicate significance level.