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. Author manuscript; available in PMC: 2010 Mar 9.
Published in final edited form as: J Immunol. 2008 Apr 15;180(8):5448–5456. doi: 10.4049/jimmunol.180.8.5448

FIGURE 7.

FIGURE 7

The CD28 pathway is not required for CD4+ NKT cell survival. A, Spleen cells from naive BALB/c or CD28−/− mice were stained with α-GalCer-loaded tetramer and mAbs against Vβ and CD4. The number of total iNKT cells and iNKT cell subsets was calculated. Results are shown as the number of cells per spleen. Data are the mean ± SEM of five mice. B, Spleen cells from CD28−/− mice were stained with α-GalCer-loaded PE-conjugated CD1d tetramers, anti-TCR Vβ, and anti-CD4. ICOS expression was analyzed by gating on CD4+ (thick line) or DN (thin line) iNKT cells. Shaded histogram represents the isotype control. C, Spleen cells from naive BALB/c (dotted line), B7-1−/−2−/− (thick line), and ICOSL−/− (thin line) mice were stained with α-GalCer-loaded PE-conjugated CD1d tetramers, anti-TCR Vβ. CD28 expression was analyzed by gating on iNKT cells. Shaded histogram represents the isotype control.