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Bright dual-color fluorescence in pCX-YNC embryos and mice. (A) The S1C2/EGFP transgenic line shows strong, widespread expression of EGFP in embryonic day 9.5 (E9.5) embryos, 2-day-old pups (P2), and adult mice. (B) The E10/YNC transgenic line illustrates strong, widespread coexpression of EYFP and ECFP in ES cells, blastocysts (E3.5), E12.5 embryos, and 4-day-old pups (P4). Selected stages are shown, both lines showed ubiquitous expression throughout development and as mice.
