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. 2010 Feb 16;6:347. doi: 10.1038/msb.2010.5

Figure 2.

Figure 2

Feedback signalling through TP53-CDKN1A-GADD45A-MAPK14-GRB2-TGFBRII-TGFβ induces ROS production and maintains DDR. (A–C) MRC5 cells were transfected with wtTP53 (pC-p53), empty vector (pCDNA), siRNA as indicated, inhibitor treated or untreated (N). DHR fluorescence intensity (black) and γH2AX foci frequency (red) were measured in unirradiated cells and at 48 h after 20 Gy IR (M±s.e.m., n=3). *P<0.04, **P<0.01, ***P<0.001 (ANOVA/Tukey), NS, not significant. (D) Pathways connecting CDKN1A to TGFβ through MAPK14. Edge thickness: LLS for any individual interaction; edge colour: pathway LLS; node colour: log fold mRNA change in senescent versus young MRC5; arrows: genes blocked in (B) and (C); grey: most probable pathway.