Figure 6.

Western blots of protein extracts of transformed cells of H. polymorpha synthesizing hybrid proteins, which combine the mature subunit of gMDH with different hsp70 presequences: the short wild-type version (lanes 1 and 2), the short version with the mutant RT → DD PTS2 (lane 5) and the long version (lane 6). The cells are grown in the presence of (NH₄)₂SO₄ (AS) or methylamine (MA) as N-source. In each lane 40 μg of protein was loaded. The blot was decorated with a-gMDH antibodies. Protein extracts of transformed H. polymorpha cells synthesizing watermelon pre-gMDH (lanes 4 and 8) or gMDH (lanes 3 and 7) provide molecular mass markers (in kDa).