Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2010 Mar 9.

Published in final edited form as: Diabetologia. 2008 Apr 18;51(6):978–988. doi: 10.1007/s00125-008-1002-3

Chronic insulin secretion during a 48hr culture is significantly decreased in isolated islets from Alox5^−/− mice compared to WT (a). Insulin secretion is defined as the amount secreted into the media and expressed as a ratio of secreted insulin to insulin content. Glucose-stimulated insulin secretion is impaired in Alox5^−/− (filled bars) vs. WT (open bars) islets (b). Basal insulin secretion was measured after a 60min incubation in KRB with 2.8mmol/l glucose and stimulated insulin secretion was measured at 5, 10, 15, 30 and 60min after changing the medium to KRB with 16.7mmol/l glucose. Compared to WT, Alox5^−/−islets have a reduced stimulatory index (c), which was calculated by dividing stimulated insulin release by basal insulin release. Despite having larger islets, the insulin content of Alox5^−/− islets, expressed per beta-cell area, is lower compared to WT (d). Expression levels of the insulin (filled bars) and Pdx1 (open bars) genes are decreased in isolated islets from Alox5^−/− mice compared to WT, as assessed by quantitative real-time quantitative PCR (e). The mRNA levels of each gene were measured in triplicate, normalized to an endogenous control, and are expressed in relative units (ru). All data are from three independent experiments from three litters of 12wk-old male Alox5^−/− and WT mice (n = 10 for each genotype). Cell culture results are from three independent experiments from three dishes for each genotype group with 20 islets per dish. Total RNA was isolated from 50 islets of each genotype, with 10 mice in each group. Data are shown as mean + SE. *P < 0.05 between Alox5^−/− and WT mice.

Chronic insulin secretion during a 48hr culture is significantly decreased in isolated islets from Alox5^−/− mice compared to WT (a). Insulin secretion is defined as the amount secreted into the media and expressed as a ratio of secreted insulin to insulin content. Glucose-stimulated insulin secretion is impaired in Alox5^−/− (filled bars) vs. WT (open bars) islets (b). Basal insulin secretion was measured after a 60min incubation in KRB with 2.8mmol/l glucose and stimulated insulin secretion was measured at 5, 10, 15, 30 and 60min after changing the medium to KRB with 16.7mmol/l glucose. Compared to WT, Alox5^−/−islets have a reduced stimulatory index (c), which was calculated by dividing stimulated insulin release by basal insulin release. Despite having larger islets, the insulin content of Alox5^−/− islets, expressed per beta-cell area, is lower compared to WT (d). Expression levels of the insulin (filled bars) and Pdx1 (open bars) genes are decreased in isolated islets from Alox5^−/− mice compared to WT, as assessed by quantitative real-time quantitative PCR (e). The mRNA levels of each gene were measured in triplicate, normalized to an endogenous control, and are expressed in relative units (ru). All data are from three independent experiments from three litters of 12wk-old male Alox5^−/− and WT mice (n = 10 for each genotype). Cell culture results are from three independent experiments from three dishes for each genotype group with 20 islets per dish. Total RNA was isolated from 50 islets of each genotype, with 10 mice in each group. Data are shown as mean + SE. *P < 0.05 between Alox5^−/− and WT mice.