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. 2003 Nov 13;100(24):14181–14186. doi: 10.1073/pnas.2336094100

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Fig. 3. — IL-18 vaccination inhibits IFN-γ production in lpr LN cells. LN cells from control mice (BALB/c, C57BL/6, MRL/Mp + ^lpr) and 16-week-old MRL/Mp lpr mice (untreated, treated with pEF2, vaccinated with pEF2-IL-18) were analyzed cytofluorimetrically after staining with phycoerythrin-labeled anti-CD4 antibodies followed by intracellular staining with a FITC-labeled anti-murine IFN-γ antibody. Results are from gated CD4⁺ LN cells from individual mice (representative of three to eight animals tested in each group) in a single experiment. Dark areas: control without antibody. Clear areas: with anti-IFN-γ antibody.