Table 1.

Spatial distribution of miRNAs in fifth-instar day 3 larvae

No.	miRNA	Probe sequence (5'-3')	HD	BW	ASG	PSG	MG	FB	OV	TE	HC	MT	Nor
1	miR-124	TAAGGCACGCGGTGAATGCCAAG	+	+									+
2	miR-1497	GCCCACCACCTGCTGCATGTA	+										+
3	miR-286	AGCACGAGTGTTCGGTCTAGTCA		+								+	NA
4	miR-263b	CTTGGCACTGGGAGAATTCAC	+										+
5	anti-miR-276-5p	AGCGAGGTATAGAGTTCCTACGT		+								+	NA
6	miR-5	CATATCACAACGATCGTTCCTTT		+								+	NA
7	miR-282	ACAGACAAAGCCTAGTAGAGGCTAGATT		+								+	NA
8	miR-29b	GCACTGAATTCGAATGGTGCTA		+						+		+	+
9	miR-283	CCCAGAATTACCAGCTGATATTTA		+			+					+	+
10	miR-7	ACAACAAAATCACTAGTCTTCCA	+	+					+	+			+
11	miR-9a	TCATACAGCTAGATAACCAAAGA	+	+					+				+
12	miR-228	CCGTGAATTCTTCCAGTGCCATT	+	+	+	+	+			+		+	+
13	miR-92	GGACTCCCTACTAGAGTCAATTT		+					+			+	+
14	miR-288	CATGAAATGAAATCGACATGAAA	+	+								+	+
15	miR-274	ATTACCCGTTAGTGTCGGTCACAAAA			+	+						+	+
16	miR-278	AAACGGACGAAAGTCCCACCGA	+	+								+	NA
17	miR-13a	CCACATCAAAGTGGCTGTGATA	+	+								+	+
18	miR-275	CGCGCGCTACTTCAGGTACCTGA	+	+					+		+	+	+
19	miR-10b-3p	ACCTCTCTAGAACCGAATTTGT	+	+			+					+	+
20	miR-10b-5p	ACAAATTCGGATCTACAGGGT	+	+			+					+	+
21	miR-133	CTACAGCTGGTTGAAGGGGACCAAATG	+	+				+				+	+
22	miR-1	CTCCATACTTCTTTACATTCCA	+	+			+	+				+	+
23	miR-277	TGTCGTACCAGATAGTGCATTTA	+	+	+	+						+	NA
24	miR-iab-4-5p	TCAGGATACATTCAGTATACGT		+			+	+	+	+		+	+
25	miR-279	TCAATGAGTGTAGATCTAGTCA	+	+				+	+	+	+	+	+
26	miR-281-3p	ATAAAGAGAGCAACTCCATGACA	+	+	+	+	+					+	+
27	miR-281-5p	ACTGTCGACGGATAGCTCTCTT	+		+	+	+					+	+
28	miR-252	CCTGCGGCACTAGTACTTAGGAA	+	+				+	+	+		+	+
29	miR-307-3p	TCACAACCTCCTTGAGTGAG
30	miR-307-5p	ACATCACACCCAGGTTGAGTGAGT	+	+				+	+		+	+	NA
31	miR-12	ACCAGTACCTGATGTAATACTCA	+	+	+	+	+					+	NA
32	miR-184-3p	GCCCTTATCAGTTCTCCGTCCA	+	+			+	+	+	+	+	+	+
33	miR-31a	ACAGCTATGCCGACTTCTTGCCT	+	+	+	+	+	+	+	+		+	+
34	miR-305	CAGAGCACCTGATGAAGTACAAT	+	+	+	+	+		+	+	+	+	+
35	miR-276-3p	AGAGCACGGTATGAAGTTCCTA	+	+	+	+	+	+	+		+	+	+
36	miR-276-5p	AGCGAGGTATAGAGTTCCTACGT		+									NA
37	miR-289	AGTCGCAGGCTCCACTTAAATATTTA	+	+	+	+	+	+	+	+	+	+	+
38	let-7a	TACTATACAACCTACTACCTCA	+	+	+	+	+	+	+	+	+	+	+
39	miR-100	CACAAGTTCGGATTTACGGGTT	+	+	+	+	+	+	+	+	+	+	+
40	miR-8	GACATCTTTACCTGACAGTATTA	+	+	+	+	+	+	+	+	+	+	+
41	bantam	AATTAGCTTTCACAATGATCTCA	+	+	+	+	+	+	+	+	+	+	+
42	miR-200b	CATCTTTACCTGACAGTATTAGA	+	+	+	+	+	+	+	+	+	+	+
43	miR-2a	GCTCATCAAAGCTGGCTGTGATA	+	+	+	+	+	+	+	+	+	+	+
44	miR-317	ACTGAGATACCACCAGCTGTGTTCA	+	+	+	+	+	+	+	+	+	+	+
45	miR-79	ATGCTTTGGTAATCTAGCTTTA	+	+	+	+	+	+	+	+	+	+	+
46	miR-34b	CAACCAGCTAACCACACTGCCA	+	+	+	+	+	+	+	+	+	+	+
Total number of expressed miRNAs			36	35	19	19	22	19	22	19	15	40	36

All expressed miRNAs confirmed by microarray and Northern blotting methods are summarized in this table. A normalized signal value ≥1,000 was regarded as the positive expression threshold. Nevertheless, only those having signals higher than 1,000 in at least four specimens are listed here as the confirmed members. The same tissues from females and males are unified here. The alpha-numeric order in the left most column is listed sequentially for easier comparison of the results in this file with the descriptions in the text. Abbreviations: +, expressed; -, not expressed; No. the alpha-numeric number; HD, head; BW, body wall; ASG, anterior silk gland; PSG, posterior silk gland; MG, midgut; FB, fat body; OV, ovary; TE, testis; HC, hemocyte; MT, malpighian tubule; Nor, Northern blotting; NA, not assayed.