Table 3.
Sequences of PCR primers1.
| Primer | Sequence (5' → 3') | |
|---|---|---|
| Histone | forward | ACT TGC TAC AAA AGC CGC TC |
| reverse | ACT TGC CTC CTG CAA AGC AC | |
| Ubiquitin | forward | AGA TCC AGG ATA AGG AAG GCA T |
| reverse | GCT CCA CCT CCA GGG TGA T | |
| PPARα | forward | GGA TGT CCC ATA ACG CGA TTC G |
| reverse | TCG TGG ATG ACG AAA GGC GG | |
| PPARγ | forward | CTC CAA GAG TAC CAA AGT GCA ATC |
| reverse | CCG GAA GAA ACC CTT GCA TC | |
| SREBP1 | forward | CCA GCT GAC AGC TCC ATT GA |
| reverse | TGC GCG CCA CAA GGA | |
| TNFα | forward | CCA CGT TGT AGC CGA CAT C |
| reverse | CCC TGA AGA GGA CCT GTG AG |
1 Primers were designed using Primer3 online software and synthesized by metabion international AG (Martinsried, Germany).