Table 7.
ΔCq1-values (mean ± SD) of hepatic mRNA expression of the genes PPARα2, PPARγ3, SREBP14, TNFα5in CLA supplemented cows vs. control group before (week -1), during (week 2 and 4) and after (week 8) treatment6.
| Control (n = 5) | CLA (n = 5) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| week -1 | week 2 | week 4 | week 8 | week -1 | week 2 | week 4 | week 8 | |||||||||
| mean | SD | mean | SD | mean | SD | mean | SD | mean | SD | mean | SD | mean | SD | mean | SD | |
| ΔPPARα | 1.5 | 0.3 | 1.4 | 0.2 | 1.9 | 0.3 | 1.7 | 0.5 | 2.0 | 0.1 | 1.4 | 0.3 | 1.9 | 0.7 | 2.0 | 0.5 |
| ΔPPARγ | 7.8 | 0.8 | 9.6 | 2.3 | 9.8 | 2.4 | 9.4 | 0.8 | 9.1 | 0.3 | 8.6 | 0.2 | 9.2 | 0.7 | 9.2 | 0.2 |
| ΔSREBP1 | 2.3 | 1.6 | 4.0 | 2.9 | 4.0 | 1.8 | 4.2 | 1.6 | 3.0 | 0.2 | 3.7 | 0.7 | 4.1 | 1.2 | 3.1 | 0.5 |
| ΔTNFα | 9.3 | 0.6 | 8.9 | 0.4 | 9.4 | 0.7 | 9.4 | 1.2 | 8.5 | 0.4 | 8.0 | 1.1 | 8.5 | 1.5 | 8.4 | 0.4 |
1 ΔCq-values were calculated as ΔCq = Cqtarget gene - meanCqrefence genes
2 peroxisome proliferator-activated receptor-alpha.
3peroxisome proliferator-activated receptor-gamma.
4sterol regulatory element-binding protein-1.
5tumor necrosis factor-alpha.
6CLA supplemented timeframe: day 1 till day 28 of lactation; five primiparous cows received a special supplement including rumen-protected CLA (10 g c9,t11-CLA/day and t10,c12-CLA/day).