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. Author manuscript; available in PMC: 2010 Mar 9.
Published in final edited form as: Mol Cancer Res. 2008 Jul 30;6(8):1365–1374. doi: 10.1158/1541-7786.MCR-08-0040

FIGURE 5.

FIGURE 5

Downstream hypermethylation and increased expression of Cacna1a during TRAMP tumor progression. A. Expression of Cacna1a in normal prostates and TRAMP tumor samples. mRNA copy number is normalized relative to 18s rRNA. Dots represent individual samples and horizontal bars indicate the mean of each sample group. Mann-Whitney test p-value: ** p < 0.006, for each group compared to normal prostate. B. Top: diagram of the Cacna1a locus. Right arrow, transcriptional start site; open rectangles, exons; lines, introns; vertical arrow, position of the NotI site identified by RLGS; black bar, CpG island; horizontal line with circles (A and B), regions analyzed by sodium bisulfite sequencing. Bottom: sodium bisulfite sequencing data of regions A and B in normal prostate and TRAMP samples. Percent methylation (averaged over the sequenced entire region), from at least ten sequenced clones per sample, is plotted. Asterisks are shown above samples that displayed ≤ 1 % methylation.