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. 2010 Mar 10;5(3):e9618. doi: 10.1371/journal.pone.0009618

Figure 6. NEDD1 L642Q mutant exists as a helical tetramer.

Figure 6

(A) Analysis of the oligomeric state of NEDD1 CTD WT, L642Q, triple 3xLQ and the YS (Y636A/S637A) mutant NEDD1 by MALS. The predicted molecular mass of these truncated constructs is 10.5 kDa. The MALS data shows a molecular mass of approx 40 kDa for each construct suggesting that the WT and the mutant proteins are tetrameric in solution. (B) Analysis of NEDD1 CTD WT and the mutants by CD spectroscopy suggests these proteins are predominantly α-helical (spectra has minima at 208 and 222 nm).