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. 2010 Mar 10;5(3):e9620. doi: 10.1371/journal.pone.0009620

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Rodriguez-Carres et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Two of the genes from F. depauperata, STE20 and MYO2, hypothesized to be ancestrally acquired in the pheromone/pheromone receptor locus of the pathogenic Cryptococcus species, clustered with the STE20 a and MYO2 a alleles of C. gattii and C. neoformans. The remaining genes from F. depauperata exhibit a species-specific phylogeny. Genes from the MAT a alleles are shown in green, and genes from the MATα allele are shown in blue. (α) indicates strains with the MATα locus, and (a) indicates strains with the MAT a locus. The tree was constructed using Maximum Parsimony, and numbers indicate total changes in the branches. Statistical support was calculated from 1,000 bootstrap replicates. Bootstrap values were >70% in all branches (values not shown). Unrooted trees for Maximum Parsimony and Maximum likelihood are shown in supporting File S1.