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. 2010 Mar 10;5(3):e9625. doi: 10.1371/journal.pone.0009625

Figure 4. Inhibition of carbonic anhydrase activity of CAIX by the anti-CAIX scFvFc antibodies.

Figure 4

The ability of the anti-CAIX scFvFc antibodies to inhibit the reaction CO2+H2O → H2CO3 catalyzed by CAIX (CAIX) was assessed using the electrometric assay as described in Experimental Procedures. Different molar ratio of antibody to CAIX enzyme was tested. The anti-CXCR4 scFvFc X33 and carbonic anhydrase inhibitor acetazolamide (AZT) were used as negative and positive control, respectively. Percent inhibition of CAIX enzymatic activity was calculated per formula discussed in the Experimental Procedures. A. Inhibition of CAIX enzymatic activity by anti-CAIX-scFvFc at molar ratio 25∶1. B. Inhibition of CAIX enzymatic activity by selected anti-CAIX-scFvFc at molar ratio 1∶1, 5∶1, and 25∶1. C. Inhibition of CAIX enzymatic activity by selected anti-CAIX-scFv antibodies at molar ratio 1∶1, 5∶1, and 25∶1. Values represent an average of three different experiments.