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. 2010 Mar 10;5(3):e9625. doi: 10.1371/journal.pone.0009625

Figure 6. Antibody-induced CAIX internalization as analyzed by ImageStream.

Figure 6

A. Single cells expressing EEA1 and Lamp1 were gated (R2), and the CypHer5E intensity was plotted for cells incubated with anti-CAIX G37, 119, or 36 (left, center, and right plots), with the relative percentage of CypHer5E+ events (R3) displayed in the upper right of each plot. Representative Brightfield, EEA1 (green), LAMP1 (orange), and CypHer5E (red) cell images from the three antibody treatment groups are shown below in the same order. B. The number of EEA1 or LAMP1 small punctate staining regions is plotted for CypHer5E+ (R3) or CypHer5E- cells from the indicated antibody treatment groups. The percentage of cells with greater than four spots (R4) is displayed in the upper right corner of each plot. Representative EEA1 images from the AbG36 sample of cells with zero (left), ten (center), or twenty EEA1 spots (right) are shown below the plots.