Table 1. The change of the resting membrane potential of SCN neurons induced by fluo-4 dye loading.

fluo-4 concentration	>150 µM	80∼120 µM	<60 µM
Number of neurons	n = 8/8	n = 9/18	n = 10/10
Initial Vm (mV)	−43.7±1.6	−43.4±0.8	−43.2±2.1
Steady state Vm (mV)	Unstable	−46.3±1.3*	−41.7±2.0

‘Initial’ and ‘steady state’ refer to the first 30 seconds of dye loading and the time just before the emergence of the first Ca²⁺ spike, respectively. The given values represent mean±SEM.

*p<0.05, t-test.