Table 1. Percentages of frameshift mutations detected in progeny of dG-, PdG, and M1G-adducted M13MB102–1 transformed into WT or repair-deficient E. coli strains.
dG* | M1G-1 | M1G-2 | M1G-3 | M1G-4 | dG | PdG-1 | PdG-2 | PdG-3 | PdG-4 | |
---|---|---|---|---|---|---|---|---|---|---|
LM102† | <0.03 | 0.10 | 0.2 ± 0.2 | 0.4 ± 0.3 | 0.2 ± 0.1 | <0.05 | <0.03 | <0.03 | <0.03 | 0.04 ± 0.03 |
LM103 (uvrA–) | 0.02 | 0.4 ± 0.3 | 0.4 ± 0.1 | 1.0 ± 0.3 | 0.5 ± 0.2 | <0.03 | 0.5 ± 0.3 | 0.10 ± 0.01 | 0.20 ± 0.06 | 0.30 ± 0.07 |
Sequence designations are as follows: 5′-GGTGTCCG1CG2CG3CG4GCATG-3′, where Gn = dG, M1G, or PdG
Results are expressed as mutation frequencies ± SD × 102 and were obtained by screening 1,000 plaques per experiment for each probe. Values are the average of three independent constructions, transformations, and hybridizations