Figure 4.

Immature particles were not detected in virus preparations. Equivalent genome copies of WNV^C6/36 and WNV^BHK were electrophoresed on a 12% Tris-HCl gel under denaturing conditions. Following transfer to PVDF membrane, membranes were incubated with antibodies against M, followed by horseradish peroxidase conjugated-goat-anti-rabbit. Protein bands were visualized using chemiluminescent detection kit. Supernatants from mock-electroporated cells were negative controls, and a lysate from Vero cells infected with WNV^BHK was a positive control for the detection of prM and M proteins. Three independent experiments were performed, and a representative western blot is shown.