Table 1.
Mortality and infectivity of WNVC6/36 and WNVBHK were indistinguishable in mice.
| Inoculum | Virus dose (PFU) | No. mice per group | Mortality (no. dead/total) | a Seroconversion (no. seropositive/survivors) | b Infection (no. infected/total) | Actual mortality (no. dead/no. infected) |
|---|---|---|---|---|---|---|
| WNVC6/36 | 1 | 12 | 1/12 (8%) | 5/11 (45%) | 6/12 (50%) | 1/6 (17%) |
| WNVC6/36 | 10 | 12 | 3/12 (25%) | 9/9 (100%) | 12/12 (100%) | 3/12 (25%) |
| WNVBHK | 1 | 11 | 1/11 (9%) | 5/10 (50%) | 6/11 (55%) | 1/6 (17%) |
| WNVBHK | 10 | 11 | 3/11 (27%) | 7/8 (88%) | 10/11 (91%) | 3/10 (30%) |
Adult, female C57BL/6 mice were inoculated SC with 1 or 10 PFU of WNVC6/36 or WNVBHK in the left rear footpad. They were observed for clinical signs and weighed daily.
a
Sera from mice that survived infection were tested for antibodies against WNV proteins by an ELISA.
b
Number of infected mice equals mice that died or were euthanized due to WN disease and surviving mice that seroconverted.