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. Author manuscript; available in PMC: 2011 Apr 25.
Published in final edited form as: Virology. 2010 Apr 25;400(1):8–17. doi: 10.1016/j.virol.2009.12.015

FIG. 1.

FIG. 1

Replication of chimeric DEN2V/YFV viruses. (A) Schematic representation of the viral genomes. YFV-specific sequences are indicated by open boxes. DEN2V-specific sequences are indicated by filled boxes. FMDV 2A indicates position of FMDV 2A protease. (B) Replication of chimeric viruses in BHK-21 cells electroporated with 5 μg of in vitro-synthesized RNA. Electroporated cells were seeded into 100-mm dishes and incubated at 37°C. At the indicated time points, media were replaced, and titers of the recombinant viruses were determined by plaque assay on BHK-21 cells as described in the Materials and Methods. Dashed line indicates the limit of detection.