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. Author manuscript; available in PMC: 2011 Apr 25.
Published in final edited form as: Virology. 2010 Apr 25;400(1):8–17. doi: 10.1016/j.virol.2009.12.015

FIG. 2.

FIG. 2

Packaging of different PIV genomes using CYF/YFV/Cherry helper. The in vitro-synthesized PIV and helper RNAs were transfected into BHK-21 cells by electroporation (see Materials and Methods for details). Cells were seeded into 100-mm dishes in 10 ml of media and incubated at 37°C. At the indicated time points, media were replaced and titers of packaged PIV and helper genomes were measured by infecting naïve BHK-21 cells with different dilutions of the samples and evaluating the numbers of GFP- and Cherry-positive cells. YFVsp indicates YFV-specific signal peptide. CYFco indicates position of YFV-specific, synthetic capsid-coding sequence, having optimized codon usage. FMDV 2A indicates positions of FMDV-specific 2A protease. YFV-specific sequences are indicated by open boxes. WNV- and DEN2V-specific sequences are indicated by filled, grey boxes. Dashed line indicates the limit of detection. (A) The results of DEN/YFV/GFP PIV and helper genome packaging. (B) The results of WN/YFV/GFP PIV and helper genome packaging. (C) The results of YF/YFV/GFP PIV and helper genome packaging.