Table 2.
Histological measures of bladder tissue in adult rats that received neonatal treatments
| Measure | Neonatal Zymosan (n=6) |
Neonatal Anesthesia (n=6) |
|---|---|---|
| # urothelial layers1 | 3.1 ± 0.3 | 3.0 ± 0.2 |
| Urothelial thickness (mm)1 | 0.033 ± 0.004 | 0.032 ± 0.003 |
| Laminae Propria thickness (mm)1 | 0.023 ± 0.003 | 0.023 ± 0.002 |
| Submucosa thickness (mm)1 | 0.071 ± 0.006 | 0.074 ± 0.006 |
| Muscularis thickness-midsection (mm)1 | 0.60 ± 0.05 | 0.57 ± 0.03 |
| Muscularis thickness-base (mm)1 | 0.76 ± 0.04 | 0.73 ± 0.04 |
| Percent connective tissue - midsection2 | 42.4 ± 2.5 | 42.9 ± 2.2 |
| Percent connective tissue - base2 | 47.3 ± 2.1 | 48.9 ± 2.0 |
| Mast cells-midsection-total (#/slice)3 | 26 ± 4 | 24 ± 2 |
| Mast cells-base-total (#/slice)3 | 38 ± 7 | 29 ± 5 |
| Mast cells-midsection-lamina propria (#/slice)3 | 9 ± 1 | 8 ± 2 |
| Mast cells-base-lamina propria (#/slice)3 | 9 ± 1 | 8 ± 1 |
Values are Mean ± SEM; there were no statistical differences between groups
All measurements performed by observer blinded to treatment group on photo images using NIH Image J software. Additional methods described in text.
1
measured in Hematolylin & Eosin stained slides
2
measured in Gomori's Trichrome stained slides
3
measured in Toluidine Blue stained slides