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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Fungal Genet Biol. 2010 Jan 12;47(4):288–296. doi: 10.1016/j.fgb.2010.01.001

Fig.1. Strategy for construction of SPT6 reintegrant strain.

Fig.1

Because transformation of the Δspt6/Δspt6 mutant was inefficient, the heterozygous strain, JMB220, was transformed with the construct depicted. Homologous recombination (depicted by “X”) resulted in a truncated SPT6 gene (SPT6*) adjacent to the native promoter (PNative), and a full length gene adjacent to the MET3 promoter (PMET3). The SAT1 gene was included in the construct to allow for positive selection of transformants. See Materials and Methods for details.