Figure 5. Comparison of peripheral T cell subsets in HSC-engrafted newborn IL2rγ^null strains.

Newborn mice of the indicated strains were irradiated and engrafted with CD34⁺ cells via IC injection, as described in Materials and Methods (Protocol B). (A) The CD4 to CD8 ratio of human CD3⁺ T cells was determined in the spleens of engrafted mice. Solid lines represent the median value for each group. Each point represents an individual animal. (B) Putative T-regulatory cell populations were identified in the spleen of each group by staining for FoxP3 and CD25. For analysis, samples were gated on CD4⁺ cells and representative dot plots are shown in panel B.