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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Fungal Genet Biol. 2010 Jan 4;47(4):310–317. doi: 10.1016/j.fgb.2009.12.010

Figure 1. Deletion of the C. neoformans BIM1 ORF.

Figure 1

A. bim1Δ∷NAT strains. Schematic of BIM1 locus before and after deletion with the NAT construct are shown with KpnI restriction sites. Southern blot reveals a 5 kb wild type band in lane 1, and a 2 kb deletion band in both a and α strains in lanes 2 and 3 respectively. B. bim1Δ∷NEO strains. Schematic of BIM1 locus before and after deletion with the NEO construct are shown with XhoI restriction sites. Southern blot reveals a 4.2 kb wild type band in lane 1, and a 2.6 kb deletion band in both a and α strains in lanes 2 and 3 respectively. Representatives of multiple, independent deletion strains are shown.