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. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Curr Alzheimer Res. 2008 Jun;5(3):319–341. doi: 10.2174/156720508784533358

Fig. 2. Punctate ADDL-binding to neurons.

Fig. 2

ADDLs isolated from AD brain or prepared in vitro show identical punctate binding to neuronal cell-surface proteins. Cultured hippocampal neurons were incubated with soluble extracts of human brain or synthetic ADDLs. Immunoreactivity against ADDLs was visualized by microscopy using M93 antibody. Soluble AD-brain proteins (a), soluble control-brain proteins (b), synthetic ADDLs (c), and synthetic ADDLs pre-treated (1 h) with oligomer-specific antibody M71 (d) are shown. Small puncta distributed along neurites, are evident for AD extracts and synthetic ADDLs, but not for control extracts or antibody-preadsorbed ADDLs (Scale bar = 10 µm). Adopted with permission from [27].