Skip to main content
. Author manuscript; available in PMC: 2010 Mar 10.
Published in final edited form as: Curr Alzheimer Res. 2008 Jun;5(3):319–341. doi: 10.2174/156720508784533358

Fig. 3. Hierarchical aggregation process of HypF-N.

Fig. 3

(a) Tapping AFM images taken under liquid (height data, scan size 670 nm, Z range 20 nm) of HypF-N globular aggregates observed a few hours after the onset of the aggregation process in the presence of 30% trifluoroethanol (TFE). Scale bar = 100 nm. Inset, STM image (height data, scan size 42 nm, Z range 15 nm) of globular aggregates obtained under the same conditions, but diluted 1:50 prior to deposition onto the substrate; the globules are apparently asymmetric and tend to form a defined mutual orientation. Scale bar = 10 nm. (b) HypF-N crescents and a ring observed after 3 days of incubation in 30% TFE (scan size 5.3 µm, Z range 45 nm). Inset, high resolution image of a ring (scan sizel.9 µm, Z range 120 nm), revealing its globular components, taken after 5 days of incubation in 30% TFE. Scale bars = 400 nm. (c) Tapping-mode AFM image taken in air (height data) after three days of incubation in 30% TFE showing the co-existence of annular structures with thin and wide ribbon-like fibrils. Scan size 4.8 µm, Z range 40 nm. Scale = 500 nm. (d) Tapping-mode AFM images taken in air (height data) of mature fibrils obtained in 30% TFE showing supercoiled fibrils after eight days (scan size 3.6 µm, Z range 80 nm). Adopted with permission from [252].