Table 1.

Sequences of PCR Primers Specific to Target Genes

MyHC I (Slow)

5’ primer, 5’-CCAAGGGCCTGAATGAGGAG-3’

3’ primer, 5-GCAAAGGCTCCAGGTCTGAG-3’

product size, 81bp

MyHC IIa (Fast)

5’ primer, 5’-AAGCGAAGAGTAAGGCTGTC-3’

3’ primer, 5’-GTGATTGCTTGCAAAGGAAC-3’

product size, 150bp

MyHC IIx (Fast)

5’ primer, 5’-CCAAGTGCAGGAAAGTGACC-3’

3’ primer, 5’-AGGAAGAGACTGACGAGCTC-3’

product size, 121bp

MyHC IIb (Fast)

5’ primer, 5’-ACAAGCTGCGGGTGAAGAGC-3’

3’ primer, 5’-CAGGACAGTGACAAAGAACG-3’

product size, 121bp