Fig. 3.

FTIR absorption and second-derivative spectra in the amide regions of GmPM1 (A, B) and GmPM28 (C, D). These panels display the spectra of proteins in D₂O (black), and after fast drying (red) and slow drying (blue). The oblongs in A and D show the α-helix bands (pink) in the amide-I and -II regions, or random coil bands (dark pink) in the amide-I region. The band positions of second-derivative spectra are basically accounted for by the spectra of slow drying, except the band at 1,658 cm⁻¹ (B) and at 1,563 cm⁻¹ (B, D) which are determined by the spectra of GmPM1 proteins after fast drying and in D₂O.