Fig. 3.

PCN alters the growth phenotype of yeast V-ATPase mutants. Yeast strains with mutations in V-ATPase were followed for 21 h while shaking at 150 rpm in yeast extract/peptone/dextrose (YPD) or YPD supplemented with 100 μg/ml PCN. (A) Cell density of parental strain BY4741 according to OD₆₃₀ over time. (B) Viability of PCN-treated BY4741 and V-ATPase mutants as determined by colony-forming unit counts on YPD agar plates. Similar results were obtained from six independent experiments. Results from one representative experiment are shown.