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PCN inactivates V-ATPase and prevents vacuole acidification in lung epithelial cells. The A549 cells were treated for 1 h with 0.5 and 2 μM BA1 or with 25, 50, and 100 μg/ml PCN or DMSO as control. Treated cells were stained with 5 μg/ml AO and examined with a fluorescence light microscope. (Original magnification, ×40.)
