Figure 1.

Endogenous hPAK65 cleavage during Fas-induced apoptosis. (A) Kinetics of caspase activation in Fas-stimulated Jurkat cells. Apoptosis was induced in Jurkat cells using anti-Fas antibody, and aliquots were taken at times indicated. Caspase-3 activity was measured on fluorogenic substrate DEVD-AFC. (B) Endogenous hPAK65 cleavage in Fas-stimulated Jurkat cells. The Jurkat cell extracts aliquoted from the same preparation were analyzed for hPAK65 cleavage by immunoblotting by using hPAK65-C antibody. The full-length and cleavage products of hPAK65 are indicated.