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Specific cleavage of recombinant hPAK65 by caspases. hPAK65, cleavage-site mutant hPAK65-D212A, and PAK1 were in vitro translated and 35S-labeled, followed by incubation at 37°C for 30 min with: buffer, recombinant caspase-3, or caspase-8, +/− Z-DEVD-FMK (50 μM), as indicated. Products were separated by SDS/PAGE followed by autoradiography. Full-length hPAK65 and cleavage fragments are indicated by arrows.
