Figure 4.

JNK activation by N-terminally truncated hPAK65. HA-tagged JNK was cotransfected into COS-7 cells with various myc-tagged constructs as indicated. Cell lysates were immunoprecipitated with anti-HA antibody, and the kinase activity was assayed using GST-Jun as substrate. Equal amounts of JNK were loaded on the gel as shown by immunoblotting by using anti-HA antibody (data not shown). Data are presented as fold activation over vector control and represent the means ± SE of three individual experiments.