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α-crystallin (hspX) mRNA levels in MTB grown in vitro (A and B; gray bars), in aerosol-infected mice (A and B; black bars), and in lung specimens from TB patients (C). Ratios of hspX/16S (A) or hspX/sigA (B and C) are means ± SD (n ≥ 3). hspX mRNA could not be quantified in lung specimen 3.1 from patient 3 (C) because of a polymorphism in hspX in this patient's strain that interfered with QRT-PCR.
