Table 3.
Antibodies against CRH, CRHBP, CRHR-1, CRHR-2, MC-R1
| Primary antibodies | Dilution | Incubation time and temperature | Prevention of non-specific binding | Retrieval procedure | Manufacturer |
| CRH (C-20)—goat polyclonal IgG (cat# sc-1759, Lot# 1071) | 1:50 | 30 min room | – | – | Santa Cruz Biotechnology Inc., Santa Cruz, CA |
| CRHBP (C-19)—goat polyclonal IgG (cat# sc-1822, Lot# E 211) | 1:50 | 30 min room | – | – | # |
| CRH-R1 (C-20)—goat polyclonal IgG (cat# sc-1757, Lot# B 1903) | 1:50 | 30 min room | – | – | # |
| CRH-R2 (N-20)—goat polyclonal IgG (cat# sc-1826, Lot# C 0303) | 1:50 | 30 min room | – | – | # |
| MC-R1—synthetic anti-hMC-1R polycolnal antibody corresponding to the amino acids 2–18 of the N-terminal, extracellular domain of the human MC-1R | 2 μg/ml | 30 min room | Quenching of endogenous peroxidase activity; blocking with 2% bovine serum albumin | High temperature antigen unmasking pretreatment | Münster, Germany. |