Table 3. Homology of PAO1 and E. coli candidate-essential genes.

Gene class query	Gene class subject	Strict orthologues
PAO1 ORFs (5,570)	E. coli known essential genes	215
PAO1 candidate-nonessential genes (4,892)	E. coli known essential genes	82
PAO1 candidate-essential genes (678)	E. coli known essential genes	133
E. coli known essential genes (232)	PAO1 candidate-essential genes	135

Comparison of predicted translations of PAO1 ORFs to E. coli. Strict orthologues were defined as the top BLAST hit that had a match length of at least 75% of the length of the query sequence.