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A. FL CSM of thermal denaturation of ca 2 mM abatacept at pH 6 (filled circles) and pH 7.5 (open circles). Insert: Thermal FL scans 10–90 °C at pH 6, between 310–390 nm, ex wl 295 nm. B. Far-UV CD melt followed between 10 –95 °C at 217 nm for ca, 3 mM abatacept and ca. 4 mM CTLA-4 (insert) at pH 6 (filled circles) and pH 7.5 (open circles) in 10 mM sodium phosphate buffer and 25 mM NaCl.
