Table 1.

Parameters for chaotrope-and thermally-induced unfolding at pH 6 and pH 7.5 monitored by intrinsic fluorescence.

		pH 6	pH 7.5
GdnHCl	ΔG1, kJ/mol	7.1±0.6	15.6±1.2
25 °C	ΔG2, kJ/mol	33.1±1.8	44.3±0.9
	Cm1, mol/l	0.8±0.1	1.3±0.2
	Cm2, mol/l	2.9±0.2	3.6±0.1
Urea	ΔG1, kJ/mol	6.8±1.3	14.5±1.3
25 °C	ΔG2, kJ/mol	47.3±3.9	nd
	Cm1, mol/l	3.1±0.2	3.8±0.2
	Cm2, mol/l	8.6±0.3	nd
Temperature	Tm1 , °C	47.7±0.1	51.6±0.3
	Tm2 , °C	80.9±0.2	78.5±0.2

nd = not determined due to short baseline