Table 2.
(Tm in °C) | pH 6 | pH 7.5 | ||||
---|---|---|---|---|---|---|
Scanrate (°C/h) |
Tm1 | Tm2 | Tm3 | Tm1 | Tm2 | Tm3 |
15 | 48.8 | 81.2 | 100.2 | 52.7 | 79.6 | 91.6 |
30 | 50.5 | 82.2 | 100.5 | 54.2 | 81.1 | 93.8 |
60 | 52.6 | 84.9 | - | 55.8 | 83.0 | 95.5 |
90 | 54 | 85.9 | - | 57.1 | 84.3 | - |
Ea** | 297±13 | 391±14 | -* | 361±19 | 391±20 | 422±18 |
kJ/mol |
a third transition is not detected the higher scan rates at pH 6, precluding estimation of the activation energy
standard errors from fitting
dH<dHv for transition 3 → aggregation. Aggregation kinetics faster at pH 6 than pH 7.5 as Tm is less scan rate dependent at pH 6 compared to pH 7.5 (see Supplementary table 2).