. Author manuscript; available in PMC: 2010 Dec 14.

Published in final edited form as: Biochemistry. 2009 Dec 15;48(49):11724–11736. doi: 10.1021/bi900853v

Table 3.

Second virial coefficient (SVC) from light scattering and zeta potential from laser doppler velocimetry for abatacept at pH 6 and pH 7.5 in 10 mM sodium phosphate, 25 mM NaCl (ionic strength = 75 mM), at 25 °C.

Light Scattering			Laser Doppler Velocimetry

SVC_el × 10⁻⁶
pH	SVC × 10⁻⁴	Mw x10³	Zeta potential	(mL mol g⁻²)	Effective charge(Z)/Theoretical charge
	(mL mol g⁻²)	(g mol ⁻¹)	(mV)	(% of SVC)
6	−6.5±3.7	78.9±2.7	−9.3±1	2.8 (0.4 %)	−2.6/−2
7.5	−6.0±1.9	68.6±3.0	−12.8±1.2	5.2 (0.9 %)	−3.5/−14.8