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. 2010 Jan;5(1):1–3. doi: 10.4161/psb.5.1.10306

Figure 1.

Figure 1

IAA and ABP1 tools (C-terminal peptide and scFv12) reduce fluorescence of FM4-64 in the plasma membrane. Protoplasts of corn coleoptiles (isolation method in1) were preincubated for 5 min with 10 µM IAA (D-F), 1 µM C-terminal peptide (G-I) or 0.1 µM scFv12 (J-L), respectively. After preincubation, 8 µM FM4-64 was applied and protoplasts were detected up to 30 min. (A–C) control protoplast were stained with FM4-64. Typical pictures of one protoplast out of four to 14 single protoplasts is shown. The pictures were taken with a CCD camera on a CLSM (TCS SP, Leica, Wetzlar, Germany). The pictures are overlays (z-stacks) of 16 single images for the control and IAA-treated protoplasts and 20 single images for the other treatments. Image number was dependent of the size of the protoplast. Bar is equivalent to 25 µm.