Skip to main content
. 2010 Feb 28;14(1):37–43. doi: 10.4196/kjpp.2010.14.1.37

Fig. 2.

Fig. 2

Effects of L-NAME, ODQ, KT-5823, and wortmannin on phosphorylation of Akt and eNOS. (A) Representative immunoblots (left) and quantitative analyses (right) of effects of db-cGMP and L-NAME on phosphorylation of Akt and eNOS. db-cGMP and KA exhibited significantly increased Akt and eNOS phosphorylation (db, KA). However, L-NAME significantly decreased KA-induced phosphorylation of Akt and eNOS (L+KA). Further, co-treatment of db-cGMP attenuated L-NAME-mediated decrease of Akt and eNOS phosphorylation (db+L+KA). (B~D) Effects of ODQ, KT-5823, and wortmannin on phosphorylation of Akt and eNOS. Representative immunoblots (left) and quantitative analyses (right) showed that KA-induced phosphorylation of Akt and eNOS was significantly decreased with ODQ, KT-5823, and wortmannin (O+KA, KT+KA, W+KA). Data represent three independent experiments and were expressed mean±SD. **p<0.01 indicates statistically significant difference from the control group. # indicates p<0.05, ## and ++ indicate p<0.01 between indicated groups.