Fig. 4.

Effect of L-NAME, ODQ, KT-5823, and wortmannin on neuronal cell death. Representative (A) and quantitative analysis (B) of neuronal cell death using cresyl violet and TUNEL stainings. In CA3 subfield, KA-induced neuronal loss was aggravated with pretreatment of L-NAME, ODQ, KT-5823, and wortmannin in cresyl violet staining. Considerable number of TUNEL-positive neurons appeared with KA treatment within CA3 region (KA) and the number of TUNEL-positive neurons were significantly increased with pre-treatment of L-NAME, ODQ, KT-5823, and wortmannin (L+KA, O+KA, KT+KA, W+KA). Co-treatment of db-cGMP with L-NAME showed attenuation on L-NAME-induced aggravation of cell death, although not significant (db+L+KA). Inset at right upper corner shows the position of the enlarged image of CA3 region. Data represent three independent experiments and were expressed mean±SD. ^**p<0.01 indicates statistically significant difference from the KA-only treated group. Scale bar: 100 mm.