Fig. 3.

DOR or CB1 agonists induce PKA Cα translocation and CRE-Luc expression in rat PHN. (A) PHN were preincubated in the presence or absence of buffer, Nal, or AM, and then incubated in the presence or absence of 1 μM DADLE or 2 μM Met for 10 min. Cα is indicated by green staining, and the neuron-specific marker, NeuN, is indicated by red staining. (B) PHN were preincubated with or without Rp for 1.5 h, or overnight with or without PTX or QEHA before incubation with or without DADLE or Met as in A. (C) PHN were treated with subthreshold concentrations of DADLE, Met, NPA, or ethanol alone or in combination as in Fig. 2 A. Where indicated, the cells were preincubated for 1 h with 1,3-dipropyl-8 cyclopentylxanthine (DPCPX) (100 nM) or 3,7-dimethyl-1-(2-propynyl)xanthine (DMPX) (10 μM). Incubation with subthreshold concentrations of each agent alone was without effect.