Fig. 2.

Dissection of voltage-dependent components by RNAi. The slo-2 mutant was used to obtain these data so that there would be no chance of contamination from the SLO-2 current. (ai) Family of K⁺ currents obtained from a slo-2 mutant muscle cell showing only the voltage-dependent components. (aii) Family of K⁺ current traces for SHAL currents (slo-2 mutant cell treated with Shaker RNAi). (aiii) SHAKER currents (a slo-2 mutant cell treated with Shal RNAi). Cells were held at -70 mV and stepped from -70 to +60 mV in 10-mV increments. (b) G-V plots and prepulse inactivation curves for SHAL currents (○) and SHAKER currents (•). The activation parameter values were V_0.5a = 11.2 ± 1.5 mV, k_a = 14.1 ± 1.04 (n = 5); V_0.5a = 20.4 ± 2, k_a = 7.7 ± 1.1 (n = 3) for SHAL and SHAKER currents, respectively. SHAL currents inactivated with values of V_0.5i =-33.1 mV ± 1.2 and k_i = 8.3 ± 0.7 (n = 6), whereas SHAKER inactivation parameter values were V_0.5i =-6.95 ± 1.7, k_i = 5.8 ± 0.5 (n = 2). Internal concentrations of Cl^- and Ca²⁺ were, respectively, 120 mM and 10 nM.