Fig. 3.

SLO-2 is a major reserve component of the delayed outward current in C. elegans. Whole-cell currents are shown from body wall muscle cells in culture, demonstrating that SLO-2 is the major outward current in these cells. Note that the scale is changed from that in Fig. 1. so that the small amplitude of the voltage-dependent components (ai and aii) can be seen relative to the SLO-2 component (aiii). (ai) Current traces from a WT cell using low Ca²⁺ and Cl^- (10 nM and 4 mM, respectively). (aii) Current traces from a slo-2 (nf100) mutant cell using high Ca²⁺ and Cl^- internal solution (200 μM and 128 mM, respectively). (aiii) Current traces from a WT cell using high Ca²⁺ and Cl^- internal solution. (aiv) Current-voltage relationships of the currents shown in ai-aiii.(b) Whole-cell currents recorded in situ from body wall muscle of adult animals. (bi) Current-traces from a WT cell using 4 mM Cl^- internal solution. (bii) Current traces from a slo-2 nf100 mutant cell using 128 mM Cl^- internal solution. (biii) Current traces from a WT cell using 128 mM Cl^- internal solution. Free internal Ca²⁺ was 10 nM in these experiments. (biv) Comparison of current-voltage relationships of currents shown in bi-biii.