FIGURE 1.

CLU constructs and protein expression in HeLa cells following transfection. A–D, schematic representation of psCLU, ΔCLU, α-chain, and β-chain molecular constructs, respectively (top, exonic sequence; bottom, protein structure). Ct, C terminus; Nt, N terminus. E, CLU and β-TrCP homology domains. Bold and capital letter R (for Arg) represents identified IκB-α binding sites (21). Underlined sequence letters represent homologous sequences between CLU and β-TrCP. F, Western blots of cytoplasmic protein extractions after CLU construct transfection were made with anti-FLAG antibody. pFLAG-BAP was used as a control plasmid. MW, molecular weight standards.